WebApr 5, 2024 · The ATP rate index corresponds to the mitoATP production rate divided by glycoATP production rate at a given time point. qRT–PCR analysis. Total mRNA from cells was purified using RNeasy Mini Kit (QIAGEN). For qRT–PCR analysis, 0.5 µg RNA was reverse-transcribed with the SuperScript II RT kit (Life Technologies). Amplification was ... WebJan 3, 2024 · MitoATP GlycoATP TotalATP Glucose Cell Respiration H+ O 2 H 2O Glycolysis ATP ADP ATP ADP Discover how live-cell metabolic measurements provide the additional insight you need . to uncover novel targets and offer functional confirmation with increased certainty. Disease model phenotype confirmation Therapeutic relevance …
GlycoMIP – An NSF Materials Innovation Platform
WebMay 24, 2024 · In contrast, the inhibition of glycoATP production by VK3 is linked to the 80% inhibition of glycolysis, resulting in a reduced glycolytic capacity and reserve. These data, which demonstrate the VK ability to differently modulate IPEC-J2 cell energy metabolism according to the different structural features of the vitamers, can mirror VK ... WebFeb 23, 2024 · However, taking the ratio of mitoATP to glycoATP, defined herein as the ATP rate index, shows that TNFα stimulation for 24 h increased overall astrocytic ATP production (Fig. 2F) by ... tick tick boom real people
Determining Mechanisms of Mitochondrial Toxicity using …
WebJul 13, 2024 · High glucose (G) increased glycolytic ATP (glycoATP) production and (H) decreased ATP rate index (mitochondrial ATP production rate/glycoATP production rate), indicating a shift to a more glycolytic phenotype. Data analyzed by (B and C) 2-way ANOVA or (E–H) 1-way ANOVA plus Bonferroni post hoc test. All data shown are mean±SD. WebJul 31, 2024 · The most striking and surprising finding of our study came from the real-time ATP production rate assay using a Seahorse XFe 24 analyser. As the glycoATP production rate and the mitoATP production rate can be detected and calculated separately, we could understand the different ways that energy is generated between different cell types. WebGlycoMIP is one of four National Science Foundation (NSF) Materials Innovation Platforms, funded by the NSF Division of Materials Research to support materials development and innovation in the U.S. Initiated on … tick tick boom release date australia