Highly expanded clones
WebApr 20, 2024 · In this study, we quantitatively tracked the expanded T cell clones and revealed their phenotype during Pneumocystis infection using scRNA-seq coupled with scTCR-seq. We comprehensively analyzed the composition and characteristics of clonal T cells, diversity of TCR repertoire, distribution of CDR3 length, and usage patterns of TCRβ … WebJul 16, 2024 · Likewise, we observed highly expanded clones with significantly disparate affinities, as exemplified by the two most expanded clones of MS-5 (5.1 and 5.2), which …
Highly expanded clones
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Clones with a frequency over 0.5% were considered highly expanded clones (HEC). Results The absolute number of HEC was significantly higher in established RA patients (mean 4.65) and tended to be higher in symptomatic RA-FDR (mean 3.4) compared with asymptomatic RA-FDR (mean 1.55, P =0.003 and P … See more Accumulating evidence indicates that T cells are actively involved in the pathogenesis of RA [1]. The current understanding of RA … See more Immunosequencing of the TCR CDR3β region of all studied subjects (n = 80) yielded a total of 3 421 000 sequence reads including … See more The results of this study reveal the presence of circulating highly expanded T-cell clones in the later pre-clinical phases of RA development. We also observed a global and significant change of the circulating TCR … See more WebDec 14, 2024 · Clones that tended to contract were more likely to exhibit an exhausted phenotype, while the only clone that remained highly expanded and exhibited markers of cytotoxicity was likely CMV-reactive. Clones appearing in the ACT product that were not abundant in the baseline tumor might be activated pharmacologically with inhibitors of PD …
Webfraction of the infected cells are from expanded clones(Fig.2,tablesS2andS3,andfig.S2).We validated the method for identifying expanded clones by making two completely independent libraries from cells from the last time point from patient 1; the same highly expanded clones were identified in both libraries. In some cases, clonal expansion was ... WebFeb 2, 2024 · It has been previously demonstrated that highly expanded clones upregulate effector molecules such as Nkg7, Ccl5, and granzymes (12, 13). Therefore, we first focused our analysis on the 30 most expanded clones for each infection by quantifying the fraction of cells present in each transcriptional cluster . Cells arising ...
WebThe ratio of highly expanded clones (HECs) was significantly higher in non-tumor tissues than in tumor tissues when the HEC threshold was defined as 2% or greater (p < 0.05). Our analysis of the median Morisita-Horn index indicated weak TCR repertoire similarity between tumor and matched non-tumor tissues. The median number of shared clones in ... WebJan 19, 2024 · Incorporating T cell receptor and transcriptome analysis, we show that circulating memory T cells are highly expanded, display extensive overlap between sites …
Webspecificity of expanded clones using three independent func-tional tests. The detailed analysis of the TCR sequences of the expanded clones showed both a highly personalized response and high sequence similarity across individuals, especially between twins. This convergence allowed us to develop a supervised clas-
WebHighly expanded conventional clones decrease interleukin 2 (IL-2) receptor alpha (IL2RA, or CD25) expression and increase indicators of senescence, suggesting that this is a … phoebe the birdWebOct 14, 2024 · Some highly expanded clones appeared to differentially express genes associated with cytotoxicity (such as granzyme B, perforin, ITGB1, and CCL4), compared to the non-expanded clones . In another study, a subset of clonally expanded memory T cells characterized by CD27 − CCR7 − were found in the peripheral blood of patients … ttc bathgateWebAug 5, 2016 · The ratio of highly expanded clones (HECs) was significantly higher in non-tumor tissues than in tumor tissues when the HEC threshold was defined as 2% or greater (p < 0.05). Our analysis of the median Morisita-Horn index indicated weak TCR repertoire similarity between tumor and matched non-tumor tissues. The median number of shared … phoebe the flamingoWebJun 29, 2024 · expansion for all three infections, with highly expanded clones having distinct transcriptional phenotypes relative to lowly expanded clones. Finally, we developed and utilized a bioinformatic ... analysis showed that more expanded clones (thicker lines in Figure 5B) were predominantly ttc bad nauheimWebOct 10, 2024 · Step 1. Install and run AOMEI Partition Assistant Professional. Then, go to "All Tools" > "Disk Clone Wizard". Step 2. Then It will pop up a window where you can choose a … ttc bathast staion idWebNov 20, 2024 · The detailed analysis of the TCR β sequences of the expanded clones showed both a highly personalized response and high sequence similarity across individuals, especially between twins. This convergence allowed us to develop a supervised classifier that predicted YFV-reactive TCR β sequences with high specificity. phoebe the babysitter killer queenWebClones with a frequency of over 0.5% were considered to be highly expanded clones (HEC). Results: In early RA synovium, the T-cell repertoire was dominated by 35 HEC (median, range 2-70) accounting for 56% of the TCR sequenced. The clonal dominance in the synovium was patient specific and significantly greater than in established RA (median of ... ttc badge