Phis2.1

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August undefined, 2024

Webb26 mars 2024 · The promoter sequence (1800 bp) of MdMATE8 (MDP0000175055) was inserted into the pHIS2.1 vector (Clontech, Palo Alto, CA, United States), which contained … Webb+ 5 µg pHis2.1 20 µl Note: To denature carrier DNA, heat to 95–100°C for 5 min, then cool rapidly in an ice bath. NOTE NOTE Protocol No. PT3529-1 Version No. PR7Z2445 32 3. Add competent cells and gently mix. 4. Add PEG/LiAc and gently mix. 5. Incubate at 30°C . NOTE: Mix cells every 10–15 min. www.clontech.com 50 µl 500 µl 30 min 600 µl 2.5 ml

VvWRKY30, a grape WRKY transcription factor, plays a …

http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf WebbpHIS2-hsSTAM1 (1-143) Sequences pHIS2-hsSTAM1 (1-143) Sequences (2) Addgene Sequences: Partial (1) Depositing Scientist Sequences: Partial (1) Partial Sequences … sims 7 release date

pHIS2 - lifescience-market.com

Webb14 jan. 2015 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ... WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … WebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: … sims 6 torrent

petunia transcription factor, PhOBF1, regulates flower senescence …

Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were … Webb6 feb. 2024 · Europe PMC is an archive of life sciences journal literature. rcmp lower mainlandWebb18 okt. 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit (#K1617-1). sims 5 xbox series x

"Webb15 okt. 2024 · For the protein–DNA-binding experiment, an oligonucleotide sequence containing triple tandem copies of the W-box and mW-box was synthesised, annealed, and cloned into the pHIS2.1 vector forming W-box- and mW-box-specific reporter vectors. " - Phis2.1

Phis2.1

Webb24 mars 2024 · In case of 4CRM1-pHis2.1, 4CRM1mut2-pHis2.1, and 4CRM1mut5-pHis2.1, 50 mM 3-amino-1,2,4-triazole (3-AT) was sufficient to suppress background growth of yeast colonies. DNA from the TF-only prey library in pDEST_GAD424 (Mitsuda et al. 2010) was transformed into Y1HGold containing 4CRM1-pHis2.1. WebbClose You have no items in your shopping cart.. Home; Plasmid; p53- His2 Model: PVT11251

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WebbPlasmid pHIS2-hsSTAM1 (1-143) from Dr. James Hurley's lab contains the insert phis2- STAM1(1-143) and is published in Structure. 2009 Mar 11. 17(3):406-16. This plasmid is …

WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the …

Webb16 feb. 2024 · To explore the involvement of bZIP genes in flower senescence, one up-regulated transcript encoding a putative ocs element binding factor, namely PhOBF1 (SGN accession no. Peaxi162Scf00285g00011), was identified during petunia flower senescence through transcriptome sequencing analysis [ 36 ]. WebbThe target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein and the target sequence stimulates …

Webb1 dec. 2024 · According to the hereditary mode of sterility genes, this vital agronomic trait can be further divided into cytoplasmic male sterility (CMS) and genic male sterility …

WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). sims 7-pattern leopardWebb1 dec. 2024 · d Growth of yeast cells on SD/-Trp-Leu-His supplemented with 100 mM 3-AT. pHIS2.1-ProGA2ox3 plus pGADT7 and pHIS2.1-ProGA2ox7 plus pGADT7 served as the negative controls. sims 8 release dateWebbpHIS-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … rcm plumbing llcWebbPhis2 1 Bait Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … sims 9 sims - row of prettiesWebbBait Vector Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > bait vector phis2. bait vector phis2 TaKaRa is a verified supplier TaKaRa ... rcmp mb newsWebb5 feb. 2024 · Either pGADT7-RrMYB18 or pGADT7-AtMYB46 was used to co-transform yeast strain Y187 with either the pHIS2.1-P RrCESAs or pHIS2.1-P AtCYCs reporter plasmids, respectively. DNA–protein interactions were quantified using the Matchmaker one-hybrid system (Clontech, now TaKaRa) according to the manufacturer’s instructions … simsa 4 mod sims 2 fontyWebb1 sep. 2024 · 1-Methylcyclopropene counteracts ethylene promotion of fruit softening and roles of MiERF2/8 and MiPG in postharvest mangoes. ... and pHIS2-MiPG were cultivat ed on SD/-T r p/-His for 3 days, and . sim sa active income fund