Pmcherry-gfp-lc3
Webtandem mCherry-GFP-LC3 construct in neurons through the nervous system and demonstrated efficient and accurate measurements of LC3 flux after pharmacological … WebMar 17, 2024 · GFP-LC3 has been used as a fluorescent marker for autophagosomes (AP) ... pEGFP-LC3 (Addgene 24920) was double digested with AfeI (blunt) and BamHI, yielding the 1.2 kb EGFP-LC3 segment. This segment was subcloned into pmCherry-C1 (Addgene 632524), which was opened by the digestion of SmaI (blunt) and BamHI. Experimental …
Pmcherry-gfp-lc3
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WebFUW mCherry-GFP-LC3 (Plasmid #110060) Print Enlarge View all sequences Purpose To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes … Webmcherry-GFP-Lc3 reporter cells enable flow cytometric quantification and sorting of cells based on autophagic flux. (A) Quantification of autophagic flux by flow cytometry using …
WebSep 17, 2024 · In response to H. pylori infection, the levels of LC3-II were strongly increased in both cell lines (Fig. 2b) and correlated with an increase in the autophagosome number in AGS-LC3 cells expressing the tandem-tagged mCherry-GFP-LC3 protein (Fig. 2c, d). All these results indicate that H. pylori infection induced autophagy in GC cell lines. WebmCherry-GFP-LC3 Use Lentiviral Tags Expression Mutation Promoter Availability Academic Institutions and Nonprofits only Enlarge pDEST-CMV mCherry-GFP-LC3B WT Plasmid #123230 Purpose Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter. Depositor
WebTransgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from … WebWe detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations …
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WebTransgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice. thd 500d2WebApr 4, 2024 · PC3 cells were cultured on coverslips for 24 h followed transfected with pmCherry-GFP-LC3 plasmid using Lipofectamine TM 2000 (Invitrogen, Carlsbad, CA, United States) according to the manufacturer’s instruction for another 24 h before receiving QLXZD (0–30 mg/mL) treatments. thd501nlWebJan 12, 2024 · Autophagy Formation Traced by mRFP-GFP-LC3 Double Fluorescence Assay. Chondrocytes were cultured on coverslips for 24 h and then transfected with pmCherry-GFP-LC3 plasmid utilizing Lipofectamine 2000 reagent (Invitrogen) for 24 h. Next, the cells were fixed with 4% paraformaldehyde and observed with a confocal fluorescence … thd-500d2 説明書WebJun 2, 2011 · When fused to the autophagic marker LC3, GFP fluorescence is quenched upon fusion between autophagosome and lysosome due to the low lysosomal pH, while mCherry remains fluorescent until degradation by lysosomal proteases [ 18, 24 ]. Cells were stably transfected with tandem-LC3. thd501glzWebpDEST-CMV mCherry-GFP-LC3B WT (Plasmid #123230) Print Enlarge View all sequences Purpose Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent … thd50300h4ssWeb(A) Quantification of autophagic flux by flow cytometry using reporter cells stably expressing mCherry-GFP-LC3. Using the ratio of mCherry to GFP, autophagic flux can be easily and … thd500glWeb质粒载体网zlzt.com专业提供pmCherry-GFP-LC3B-d,产品编号为zl-042263,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹,质粒合成,质粒 … thd50400h4ss