WebMar 26, 2024 · 100(聚乙二醇辛基苯基醚)是一种非离子型表面活性剂(或称去污剂)。分子量为646.86(C34H62O11)。它能溶解脂质,以增加抗体对细胞膜的通透性。 ... Triton X-100. 编辑词条. 添加义项 ... Web细胞通透缓冲液:购买即用型 或者通过添加 30 µl Triton™ X-100 至 10 ml 抗体稀释缓冲液来制备10 ml。4°C 保存。 抗体稀释缓冲液:购买即用型流式细胞术抗体稀释缓冲液 ,或在 100 ml 1x PBS 中溶解 0.5 g 牛血清白蛋白 (BSA) 来制备 0.5% BSA PBS 缓冲液。4°C 保存。
Triton X-100的性质及使用注意事项 - 百度文库
WebFor 100 mL 4% PFA fixative, dissolve 4 g of paraformaldehyde powder in 60 mL distilled water. Add 5–8 tablets of KOH until the solution turns clear as PFA is fully dissolved, and then adjust pH to 7.0 with HCl. Add 1 mL 10% Triton X-100 to reach the final concentration of 0.1% (v/v), and then add distilled water to 100 mL. WebDec 23, 2007 · Triton X-100是一种非离子型表面活性剂(或称清洁剂),分子量为646.86(C34H62O11)。它能溶解脂质,以增加抗体对细胞膜的通透性。1%的Triton X … chercher ma boite mail
流式细胞术,Triton™ X-100 通透实验步骤 Cell Signaling Technology
WebTriton X-100 常与 CHAPS 等 zwitterionic 去垢剂一起使用来纯化膜蛋白,使膜. 蛋白保持其天然构象。 4.Triton X-100 可以提高真核细胞细胞膜的通透性,因此在 Immunostaining 时 … WebProduct name : Triton™ X-100 Product Number : X100 Brand : Sigma-Aldrich CAS-No. : 9036-19-5 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses : Laboratory chemicals, Synthesis of substances 1.3 Details of the supplier of the safety data sheet Company : Sigma-Aldrich Inc. WebFeb 21, 2015 · In our experiments, Triton X 100 has an inhibitory effect for some hydrolases and oxidoreductases in the non- immobilised system. Yes, dear Mahmoud, but it depends on Triton X100 concentration, at ... chercher localisation ip